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New Lab Start Up Program 2011
Catalogs | Sciences 2011-07-18 08:45:42
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    Comprehensive Sourcing for Laboratory Solutions Volume 1 * No. 1, 2011 Canadian Edition

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    TrustedPartner Count on Fisherbrand for the products you need, when you need them. Quality, reliability and value. 1-800-234-7437 * www.fishersci.ca © 2009 Thermo Fisher Scientific Inc. All rights reserved.

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    Supplier/Product Guide ChemiCals emD Chemicals TLC Plates ............................ 6 Fisher Chemical CellPURE and JustPURE Buffers ...................................... 4, 5 Ricca Chemical Company Environmental Line ....................................... 7 ChRomatogRaphy National scientific Easy Columns .................... 7 WR grace Reveleris Flash Cartridges .............. 8 CoNsumables bel-art scienceWare PrepSafe Microcentrifuge Tube Mini Floating Racks ............................................. 16 Corning Reusable Plasticware ....................... 18 emD millipore EZ Slide ............................... 14 eppendorf North america Xplorer Electronic Pipettes ....................................... 17 Fisher scientific Traceable Hi-Accuracy Refrigerator Thermometer .......................... 13 Fisherbrand Reusable Glass Media Bottles, Superfrost Slides .......................................... 10 Fisherbrand Shaker Flasks ............................... 9 interlab supply PolySeed Capsules ............... 19 lab armor Bath Beads .................................. 19 mbp ART Reload Tips .................................. 15 thermo scientific Finnpipette, Novus Pipetter ........................................................ 15 thermo scientific BioLite Cell Culture Products ......................................... 11 thermo scientific Matrix Equalizer Pipetters ....................................................... 16 thermo scientific Nalgene Economy Bottles .......................................................... 12 Vistalab technologies Ovation Multichannel Pipettes .................................. 19 liFe sCieNCes abnova ELISA Kits and Assay Kits ................ 40 amg EVOS Microscope ............................... 20 bD biosciences Tools for Assessing Cellular Events ............................................ 22 bD biosciences Cell Culture Flasks ............... 23 bel-art scienceware Vacuum Aspirator Collection System ......................................... 40 biochrom Spectrophotometers ...................... 27 biotek instruments Synergy H1 Multimode Reader, MultiFlo Dispenser ......................... 38 biotek instruments Epoch Spectrophotometer, EL406 Washer Dispenser ........................................ 28 emD millipore Western Blotting Products ...................................................... 21 eppendorf North america Cell Culture Centrifuges .................................................. 25 eppendorf North america Mastercycler pro PCR Systems ......................................... 24 ge healthcare illustra Ready-To-Go PCR Beads ................................................... 26 ge healthcare Hyperfilm .............................. 26 thermo scientific Forma Plus Ultra-Low Temperature Freezers .................................. 36 thermo scientific Herasafe KS Biological Safety Cabinets ........................... 36 thermo scientific Pierce Agarose ChIP Kit.... 29 thermo scientific Pierce Fast Transfer ........... 29 thermo scientific Enzymes and Reagents from Finnzymes and Fermentas ............ 32, 33 thermo scientific NanoDrop Products ......... 35 thermo scientific Particle Size Standards ...... 39 thermo scientific Pierce Primary Antibodies ................................................... 30 thermo scientific Pierce Protein Ladders ...... 31 thermo scientific Piko Thermal Cycler, Evolution 260 Bio Spectrophotometer ........ 34 thermo scientific Heracell i Tri-Gas Incubators, Savant DNA 120 SpeedVac Concentrator ............................................... 37 lab equipmeNt brandtech VACUUBRAND ME1, ME1C Pumps .............................................. 43 Denver instrument Moisture Analyzer........... 51 emD millipore Milli-Q Integral Lab Water System ........................................ 53 Fisherbrand Dual-frequency Ultrasonic Cleaners ....................................................... 48 Fisherbrand Upholstered Chairs .................... 59 Fisherbrand Polymer Carts ............................ 61 Fisher scientific Sonic Dismembrators, accumet Universal pH/ATC Electrodes ....... 42 Fisher scientific Isotemp Plus Lab Refrigerators and Freezers ................................................. 41 Fisher scientific Isotemp Thermal Mixers .................................................................... 60 Fisher scientific PowerGen Homogenizers .... 43 Foss Soxtec Extraction Systems ..................... 55 hamilton MICROLAB 600 ........................... 58 heidolph brinkmann Hei-VAP Rotary Evaporators ................................................. 56 iKa Works Magnetic Stirrers ......................... 49 iKa Works Rotary Evaporator ...................... 50 Kinematica Polytron Homogenizer ............... 55 KNF Neuberger SC920 Vacuum Pump System ............................................... 50 labconco FlaskScrubber Glassware Washer ... 54 lamotte Turbidity Meters ............................. 57 mettler toledo Standard Solutions Preparation .................................................. 63 plas-labs Glove Boxes............................... 57 sartorious Process Efficiency in the Lab ........ 52 thermo scientific Accela HPLC System ........ 59 thermo scientific Evolution 200, Spectronic 200 ............................................ 47 thermo scientific Heratherm Incubators ...... 45 thermo scientific Heratherm Ovens, Forma Environmental Chambers ................ 46 thermo scientific Orion Star RDO Portable Meters ........................................... 48 thermo scientific MaxQ Shakers.................. 62 thermo scientific Sorvall Legend and Sorvall ST Centrifuge Series ........................ 44 lab saFety 3m VFlex Particulate Respirators .................. 66 Dupont tychem Chemical Protective Clothing ...................................................... 65 Fisherbrand Nitrile Gloves ............................. 65 Justrite Safety Containers............................... 64 Kimberly-Clark Kimtech Sterling Nitrile Exam Gloves ....................... 64 medicom Safetouch Gloves ........................... 67 North by honeywell Adaptec Eyewear System ......................................................... 66 Trademarks of our valued suppliers: Abnova, accu-jet pro, AirTite, ART, Auto-Cal, Axio, BD Biosciences, Bel-Art, BioLite, BioTek, BrandTech, Carl Zeiss, Corning, Davisil, Denver Instrument, Dispensette, DriSolv, EMD, Epoch, Eppendorf, Eppendorf Research, Eppendorf Xplorer, Evolution, EVOS, Excella, Explorer, ExSap-IT, EZ Slide, Fermentas, Finnpipette, Finnzymes, FlaskScrubber, Forma, Grace, Grace Davison Discovery Sciences, Hamilton Company, Heidolph, Hei-VAP, Herasafe, Heratherm, HybriLinker, IKA, illustra, Immobilon, Innova, iS, Kinematica, KNFLab, Lab Armor, Lab Armor Beads, Labconco, Luminata, Mastercycler, MaxQ, Mettler Toledo, MICROLAB, Millicell, Millipore, Milli-Q, Molecular BioProducts, Nalgene, NanoDrop, Nanosphere, Nicolet, Norm-Ject, Novus, Ocelot, OMNIC, One Click, One Click Titration, One Click Water Determination, Orion, Ovation, PageRuler, Pierce, Piko, Pinnacle, Plas-Labs, PolySeed, PolySeed NX, PolyTox, PowerGen, PrepSafe, PYREX, Ready-To-Go, Reichert, Reveleris, RICCA CHEMICAL COMPANY, SafetyPrime, Sartorius, Savant, Scienceware, SNAP i.d., Sorvall, SPECTRONIC, Star, Stereomaster, Steri-Cycle, Thermo Scientific, Traceable, UV Probe, VACUUBRAND, Versette, VistaLab Technologies, WTW, Zeiss Trademarks of Fisher Scientific: accumet, BioTrack, CellPURE, Fisher BioReagents, Fisherbrand, Fisher Chemical, Fisher Scientific, Isotemp, JustPURE, LabReporter Nonsupplier Trademarks: ASTM, DIN, ISO, NIST, USP For trademark ownership, see www.fishersci.com/trademarks. PHONE: 1-800-234-7437 FAX: 1-800-463-2996 ONLINE: www.fishersci.ca 3

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    CHEMICALS Fisher BioReagents CellPURE Biological Buffers Fisher BioReagents buffers meet the needs of every budget and scale. * Economical powders come in various package sizes * Concentrated stock solutions provide convenience in one step of easy dilution * Ready-to-use solutions offer the biggest time savings of all The purpose of a buffer in a biological system is to maintain intracellular and extracellular pH within the physiological range and resist changes in pH due to the presence of internal and external influences. CellPURE Biological Buffers from Fisher BioReagents are ultrapure zwitterionic buffers that possess both positive and negative charges. First described by Good and co-workers in 1966, these organic buffers have pKa values at or near physiological pH and minimal interference with biological processes. CellPURE Biological Buffers are ideal for cell cultivation, isolation of cells, enzyme assays, and other biochemical applications. (Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): 467-477) Properties of CellPURE Biological Buffers * Ultrapure zwitterionic biological buffers * Optimized for cell/tissue culture work * Wide applicability to molecular biology and biochemical studies * Manufactured under strict quality control guidelines to ensure performance and reliability Obtaining optimal results in your research requires careful selection of reagents. When your experiments require exact buffering conditions, you can depend on the reliability of Fisher BioReagents buffers. All Fisher BioReagents buffers are manufactured from high-quality raw materials under ISO 9001 2000-certified manufacturing and testing processes. In addition, buffer sterilization is done by submicron filtration and/or autoclaving. Advantages * Cell culture tested * Analyzed for the absence of nucleases and proteases * Tested for endotoxin and bioburden levels * pKa values mostly independent of temperature and concentration * High water solubility * Minimal permeability to biological membranes Applications * Tissue culture media and maintenance of cell lines * Enzyme assays * Electrophoresis of RNA * Transfection of mammalian cells Cat. No. Product Description Formula Molecular Weight pKa @ 25°C Useful pH Range Cell Culture Tested Endotoxin Assay Nuclease and Protease Tested Size BP2941-100 BIS-TRIS C8H19NO5 209.24 6.5 5.8 to 7.2 X X X 100g BP2943-100 BIS-TRIS Propane C11H26N2O6 282.33 6.8† 6.3 to 9.5 X X X 100g BP2947-100 BES C6H15NO5S 213.25 7.1 6.4 to 7.8 X X X 100g BP2936-100 MOPS C7H15NO4S 209.26 7.2 6.5 to 7.9 X X X 100g BP2946-25 MOPS Sodium Salt C7H14NNaO4S 231.25 7.2 6.5 to 7.9 X X X 25g BP2937-100 HEPES C8H18N2O4S 238.3 7.5 6.8 to 8.2 X X X 100g BP2939-100 HEPES Sodium Salt C8H17N2NaO4S 260.29 7.5 6.8 to 8.2 X X X 100g BP2945-100 TES C6H15NO6S 229.25 7.5 6.8 to 8.2 X X X 100g †pKa = 9.0 for the second dissociation stage Technical Tip The Water Makes a Difference. CellPURE Biological Buffers are of the highest Quality (purity ((≥99%). They are tested for low heavy metal content and the absence of endotoxins, nucleases, and proteases. It is important to use only water of the highest quality to prepare the buffer solutions. Water that stands too long in pipes or water produced by a system needing routine maintenance increases the risk for contamination of buffer solutions. Fisher BioReagents provides several grades of high purity water suitable for the preparation of high quality buffer solutions. 4 No. 1 * 2011

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    Fisher BioReagents JustPURE Good Buffers Zwitterionic buffers possessing both positive and negative charges were described in 1966 by Good and co-workers as being suitable for work with biological molecules. Popularly known today as "Good" buffers, these organic buffers have several advantages compared to traditional inorganic buffering systems such as phosphate, borate and bicarbonate. "Good" buffers approach the "ideal" buffer state by having pKa values at or near physiological pH and minimal interference with biological processes. JustPURE "Good" buffers from Fisher BioReagents have very high purity (assay > 99%) and only trace amounts of metal ions, which makes them useful for applications requiring tight control of elemental content. (Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): 467-477) Properties of JustPURE Buffers Cat. No. Product Description * Ultrapure zwitterionic buffers * Optimized for research in cellular and molecular biology * Low interference with biological reactions Advantages * pK a values mostly independent of temperature and concentration * High water solubility * Chemically and enzymatically stable * Minimal permeability to biological membranes * Limited effect on biological reactions * Minimal absorption in spectral range 240 to 700nm Applications * High efficiency transfection of mammalian cells * Gel electrophoresis of RNA * Protein isolation applications * Cell cultures and enzyme assays * Bioanalytical methods such as IEF, 2-D electrophoresis, SDS-PAGE CAS No. Formula Molecular Weight pK a @ 25°C Useful pH Range Form Size BP2920-250 MES Hydrate 4432-31-9 C6H13NO4S xH2O 195.24 (anhydrous) 6.1 5.5 to 6.7 White powder 250g BP2935-100 ACES 7365-82-4 C4H10N2O4S 182.2 6.8 6.1 to 7.5 White powder 100g BP2924-50 PIPES 5625-37-6 C8H1188N2O6S2 302.37 6.8 6.1 to 7.5 White powder 50g BP2929-25 BIS-TRIS Propane 64431-96-5 C11H26N2O6 282.33 6.8† 6.3 to 9.5 White powder 25g BP2925-100 MOPS 1132-61-2 C7H15NO4S 209.26 7.2 6.5 to 7.9 White powder 100g BP2926-100 MOPS Sodium Salt 71119-22-7 C7H14NNaO4S 231.25 7.2 6.5 to 7.9 White powder 100g BP2921-50 HEPES 7365-45-9 C8H18N2O4S 238.3 7.5 6.8 to 8.2 White powder 50g BP2931-100 HEPES Sodium Salt 75277-39-3 C8H17N2NaO4S 260.29 7.5 6.8 to 8.2 White powder 100g BP2933-100 EPPS 16052-06-5 C9H20N2O4S 252.33 8.0 7.3 to 8.7 White powder 100g BP2922-100 Tricine 5704-04-1 C6H13NO5 179.17 8.1 7.4 to 8.8 White powder 100g BP2930-50 Gly-Gly 556-50-3 C4H8N2O3 132.12 8.2 7.5 to 8.9 White powder 50g BP2923-100 TAPS 29915-38-6 C7H17NO6S 243.28 8.4 7.7 to 9.1 White powder 100g BP2934-100 CHES 103-47-9 C8H17NO3S 207.29 9.3 8.6 to 10.0 White powder 100g BP2928-100 CAPS 1135-40-6 C9H19NO3S 221.32 10.4 9.7 to 11.1 White powder 100g †pKa = 9.0 for the second dissociation stage PHONE: 1-800-234-7437 FAX: 1-800-463-2996 ONLINE: www.fishersci.ca 5 CHEMICALS

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    CHEMICALS TLC Plates from EMD Chemicals Getting your TLC plates from EMD not only makes a lot of sense, but gives you peace of mind that you won't be compromising your TLC results due to impurities in your mobile phase. EMD's TLC plates are available with many sorbent choices, on classical glass backing (with or without prescoring) as well as flexible plastic and aluminum formats that allow easy trimming and economical custom sizing. Preparative and high performance versions are also available, as well as many innovative specialty plates. Our Preconcentration Zone plates minimize spotting shape variations and give uniform sharp bands with higher resolution as well as serving as a sample cleanup step. All plates are available with or without a fluorescent-254 indicator that enables visualization of colorless sample components without spraying or charring. Description Plate Size Indicator Layer Thickness Cat. No. Pack of Precoated Glass Silica Gel 60 F254 2.5 × 7.5cm Yes 250µm M15341-5 500 Silica Gel 60 F 254 20 × 20cm Yes 250µm M5715-7 25 Aluminum Sheet Silica Gel 60 F 254 20 × 20cm Yes 200µm M5554-7 25 Precoated Preparative Layer Glass Silica Gel 60 F 254 20 × 20cm Yes 500µm M5744-7 20 6 No. 1 * 2011

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    Ricca Chemical Environmental Testing Line RICCA gives you the most accurate results for your Environmental testing Ready-to-use analytical solutions and standards. Test (APHA/EPA) Description Cat. No. Alkalinity Sodium Carbonate, 0.0500 Normal 7185-16 Biomedical Oxygen Demand Glucose-Glutamic Acid Solution 32-551-6 Biomedical Oxygen Demand Phosphate Buffer Solution, pH 7.2 5800-16 Chloride Silver Nitrate, 0.0141 Normal 686016 Color Color Standard, Color = 500 Units 22304 Cyanide Cyanide Standard, 1000ppm CN 2543-16 Fluoride Fluoride Standard, 10ppm F 3170-16 Fluoride Total Ionic Strength Adjustment Buffer (TISAB) 8670-16 Hardness Calcium Chloride Standard, 1mL = 1mg CaCO3 1780-16 Hardness EDTA, 0.0100 Molar 27-001-6 Hardness Water Hardness Buffer 9200-16 Nitrogen (Ammonia) Nitrogen Standard, 1000ppm NH3 5455-16 Nitrogen (Ammonia) Sodium Hydroxide, 10.0 Normal 7470-32 Oxygen (Dissolved) Alkali-Iodide-Azide Reagent, Alsterberg 540-16 Phosphorus Hydrochloric Acid, 50% (v/v) Aqueous 3580-16 Phosphorus Phosphate Standard, 50ppm P as SO4 5830-16 Sulfate Sulfate Standard, 100ppm SO4 8110-16 Total Organic Carbon (TOC) Carbon Standard, Organic, 1000ppm C 1847-16 Turbidity/Nephelometric Method Turbidity Standard, 4000 NTU 8830-16 National Scientific EASY-Column and Connectors Provide chromatographic excellence in nanoscale separations biocompatible, metal-free fused silica capillaries. each column-end is precisely cut and polished to ensure a perfect, zero dead-volume connection to emitter or transfer line. * Compatible with any nanoscale hplC system * optimized for online lC-ms * quality control on every column * simple, flexible design * Zero dead volume unions and nanoliter dead volume tees ensure minimum system dispersion Description Cat. No. 2cm; 100µm ID; 5µm C18-A1 03-052-619 10cm; 75µm ID; 3µm C18-A2 03-052-618 10cm; 75µm ID; 3µm C18-A2, pk/3 03-052-616 HPLC to column connector kit 03-052-617 Mixing/Venting Tee, 2-column kit 03-052-615 2-column setup connector kit 03-052-614 Sleeves, 2cm, 1/32 OD to 36µm 03-052-613 PHONE: 1-800-234-7437 FAX: 1-800-463-2996 ONLINE: www.fishersci.ca 7 CHEMICALS CHROMATOGRAPHY

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    CHROMATOGRAPHY Grace Reveleris Flash Cartridges Discover More Grace combines stringent quality controlled silica in a smaller particle format with our proprietary packing. This keeps back pressure low enabling greater loading capacity and savings of time and money. What Separates Our Cartridges from Others? As a world-renowned silica manufacturer and leading chromatography supplier, GRACE delivers high-performance flash cartridges packed with Davisil silicas, optimized for use in flash chromatography. The Resolv and Reveleris brand cartridges: 1) Provide higher loading capacity - meaning fewer and shorter runs 2) Resolve more components - achieve greater compound purities 3) Lower consumable costs - including waste and solvents Get the Most Out of Your Flash Applications Get the most out of your flash applications by choosing Reveleris flash cartridges, the only flash cartridges designed by a silica expert for ideal chromatographic performance. * Increased loading capacity * Durable 200psi design * Superior resolution with ultrapure 40μm silica * Four unique chemistries available Reveleris Flash Cartridges Bed Weight Mfr. No. Cat. No. SRC Silica Flash Cartridges 4g 5146130 AT5146130 12g 5146131 AT5146131 40g 5146132 AT5146132 80g 5146133 AT5146133 120g 5146134 AT5146134 330g 5146135 AT5146135 Reveleris Reversed-Phase C18 Cartridges 12g 5152103 AT5152103 40g 5152104 AT5152104 80g 5152105 AT5152105 Amino Flash Cartridges 4g 5157330 AT5157330 12g 5157331 AT5157331 40g 5157332 AT5157332 80g 5157333 AT5157333 120g 5157334 AT5157334 Diol Flash Cartridges 4g 5157335 AT5157335 12g 5157336 AT5157336 40g 5157337 AT5157337 80g 5157338 AT5157338 120g 5157339 AT5157339 8 No. 1 * 2011

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    Shaker Flasks Ideal for suspension cell culture, media preparation and storage The most economical choice. * Certified sterile at 10 -6 SAL USP Class VI * Nonpyrogenic and noncytotoxic * Autoclavable * Clear, durable polycarbonate (PC) with molded-in graduations * Polypropylene (PP) closure with 0.22µm PTFE pore * Individually packed for easy storage and handling * Plain bottom for use on benchtop or baffled bottom for shaker table use * Vented or nonvented screw closure Fisherbrand Shaker Flasks Capacity Quantity/Case Cat. No. Plain Bottom, Vented Closure 125mL 24 PBV125 250mL 12 PBV250 500mL 12 PBV500 1000mL 6 PBV1000 2000mL 4 PBV2000 2800mL 4 PBV2800 Plain Bottom, Nonvented Closure 125mL 24 PBNV125 250mL 12 PBNV250 500mL 12 PBNV500 1000mL 6 PBNV1000 Baffled Bottom, Vented Closure 125mL 24 BBV125 250mL 12 BBV250 500mL 12 BBV500 1000mL 6 BBV1000 2000mL 4 BBV2000 2800mL 4 BBV2800 PHONE: 1-800-234-7437 FAX: 1-800-463-2996 ONLINE: www.fishersci.ca 9 CONSUMABLES

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    CONSUMABLES Reusable Glass Media Bottles with Cap Convenient wide opening * Enhanced graduations * Permanent white enamel marking spot * GL 45 screw thread * Clear (natural), drip-free, replaceable polypropylene pour ring * Linerless * Autoclavable (to 140°C) * Polypropylene cap FB-800-100 Capacity Graduation Range Cat. No. 100mL 20 to 80mL FB-800-100 250mL 50 to 200mL FB-800-250 500mL 100 to 400mL FB-800-500 1000mL 100 to 900mL FB-800-1000 2000mL 400 to 1800mL FB-800-2000 5000mL 1000 to 4000mL FB-800-5000 10,000mL 2000 to 8000mL FB-800-10000 Superfrost Disposable Microscope Slides Superfrost coating impervious to most common laboratory chemicals and reagents * Brilliant white, opaque coating takes and retains most laboratory marking methods * Each side has a written key for easy orientation; the word "SPECIMEN" instantly tells you which side is up * Slides can be imprinted with your laboratory or hospital's name (call your Fisher Scientific Customer Service Representative for details) Ordering Information: Come in handy one-at-a-time dispenser boxes of 72 slides. Size Thickness Cat. No. 3 × 1" 1mm 12-550-123 75 × 25mm 1mm 12-550-143 3 × 1" 1mm 22-178-277 † † This slide undergoes additional quality control reviews. 10 No. 1 * 2011

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